Journal: bioRxiv
Article Title: Astrocytic RNA degradation suppresses calcium signaling to support synapse function and restrain anxiety
doi: 10.1101/2025.11.18.689044
Figure Lengend Snippet: ( A ) Confocal microscopy images depicting regions of interest (ROI, white boxes) highlighting Upf2 + /GFAP + astrocytes in hippocampus and cortex. ( B ) UPF2 protein is reduced in cKO mice (CTX: CTRL: 100 ± 6, cKO: 67 ± 2, p < 0.0001, Unpaired t -test; HPC: CTRL: 100 ± 5, cKO: 75 ± 1, p = 0.0003, Mann-Whitney test. n = 4 mice per genotype, 20 ROIs per group. Data is presented as mean ± S.E.M. *** denotes p < 0.005, **** denotes p < 0.001. This figure confirms the downregulation of UPF2 protein levels in hippocampus and visual cortex of cKO mice.
Article Snippet: Spinning disk confocal microscopy followed by Imaris 3D reconstructions facilitated the quantification of PSD-95 + excitatory synapses as well as engulfment of synaptic material ( ).
Techniques: Confocal Microscopy, MANN-WHITNEY